In My Little Pony Equestria Girls, Applejack first appears delivering fizzy apple cider to Pinkie Pie, the head of the Fall Formal planning committee. She tries to dissuade Twilight Sparkle from running for Princess, telling her that Sunset Shimmer viciously seeks to humiliate any competition.
It is later revealed that Applejack and the rest of Twilight's new friends had been driven apart by Sunset Shimmer's machinations. Rainbow Dash had promised to have the school's softball team attend a bake sale that Applejack was running, only for Rainbow to be messaged that the sale had been moved to a different day. Once Twilight convinces Applejack to talk to Rainbow about the misunderstanding, all five friends reconcile and help Twilight get voted for Princess of the Fall Formal.
When Sunset puts on Twilight's crown and transforms into a "raging she-demon", Applejack briefly channels the element of honesty and gains pony-like attributes.
Applejack reappears in the sequel My Little Pony Equestria Girls: Rainbow Rocks and its tie-in animated shorts, where she plays bass in her friends' band, the Rainbooms. She first appears helping her friends make a sign for the CHS Musical Showcase. At band rehearsal, she helps comfort Sunset Shimmer of all the bad memories of what happened at the Fall Formal. After they perform one song, she wonders why she and her friends gain pony ears and a longer ponytail while they perform even though Princess Twilight took her crown with her when she went back to Equestria.
Throughout the film, Applejack frequently argues with Rarity about her concerns about the band's stage costumes, finding them to be inconsequential to the band. While trapped under the Battle of the Bands stage, Applejack and her friends reconcile after hearing Sunset Shimmer's speech, and Applejack finally decides to let Rarity use her band outfits for their performances.
In Music to My Ears, Applejack appears briefly waving to DJ Pon-3 at the Sweet Shoppe with her friends. In Guitar Centered, she accompanies her friends at the music store and suggests a musical competition between Rainbow Dash and Trixiefor an expensive guitar. In Pinkie on the One, she appears as equally frustrated as Rainbow Dash and Rarity with Pinkie Pie's escalating drumming and notes Rarity's lateness to the Rainbooms' rehearsal is to make a "grand entrance" in Player Piano.
Applejack is the lead role in A Case for the Bass, wherein her bass is accidentally sold by Granny Smith to pawn shop owners Flim and Flam. She plays a skillful bass solo and transform into her half-pony form when they question her playing ability, and later works off the bass as a costumed mascot in front of their store. She performs the titular songs with the Rainbooms in Shake Your Tail and Perfect Day for Fun, respectively briefly suggesting a rodeo theme for a school dance and enjoying the Canterlot High School carnival with her friends, competing at a high striker game at one point.
In the encore short My Past is Not Today, Applejack briefly appears in a flashback putting her hand on Sunset Shimmer's after her defeat. In Friendship Through the Ages, she performs the titular song with the Rainbooms and line dances with Sunset Shimmer in one shot.
Applejack appears again in My Little Pony Equestria Girls: Friendship Games, competing in the games against Crystal Prep Academy as part of Canterlot High's Wondercolts team. She participates in the Academic Decathlon, passing the baking contest but failing the spelling bee after misspelling "onomatopoeia".
She is paired up with Fluttershy during the archery portion of the Tri-Cross Relay. When the human world's version of Twilight Sparkle is heckled by her own team for failing to hit a target, Applejack gives her advice that allows her to hit a bull's-eye. This act of honesty causes her to pony up, but her magic is drained by Twilight's amulet. She later regains her magic to empower Sunset Shimmer and return Twilight to normal after she is transformed into Midnight Sparkle.
In The Science of Magic, Sunset Shimmer attempts to figure out how magic works in the human world. When she tests it out on Applejack, she fails to pony up but somehow, apples fall from the sky, one of which Applejack eats.
In the fourth Equestria Girls film, Applejack goes with her friends and classmates to Camp Everfree, where she expresses a desire to rough it in the woods and forage for her own food. At Camp Everfree, she is paired with Rainbow Dash in the Aquamarine Tent. While at the camp, Applejack 's magic undergoes a dramatic change, and she develops the ability of superhuman strength.
Applejack comes up with the idea of building a new dock for Camp Everfree as part of her class's "gift for future campers", and she spends much of the film helping her fellow Wondercolts build it. When camp director Gloriosa Daisy is consumed by Equestrian magic and Twilight Sparkle overcomes her fears of Midnight Sparkle, Applejack helps her friends defeat Gloriosa. She also assists in saving Camp Everfree from closing by organizing a Crystal Ball fundraiser.
In the half-hour special Dance Magic, Applejack helps her friends raise money to renovate Camp Everfree. When Rarity's idea for a music video is copied by the Shadowbolts, Applejack comes up with the new idea of making a cowgirl-themed music video. She also takes part in the Dance Magic music video near the end of the special.
In the half-hour special Movie Magic, Applejack accompanies her friends to the set of the Daring Do movie, expressing an interest in seeing the volcano movie sets. She is also briefly mistaken for an actress in the Power Ponies movie, cast as Mistress Mare-velous. In the end, she helps expose Juniper Montage's thievery and deception, and she is cast as an extra in the Daring Do film.
In the half-hour special Mirror Magic, Applejack becomes trapped with her friends in limbo when Juniper Montage acquires a hand mirror enchanted with Equestrian magic. When Starlight Glimmer succeeds in convincing Juniper to let them out, Applejack and the rest of her friends forgive and accept her as a new friend.
Applejack serves as the main focus of the musical short Shake Things Up!, in which she works at a juice bar in the Canterlot Mall. Tired of serving the same drinks, she decides to "shake things up" and mix drinks with a wide variety of fruit and vegetables. Applejack is also a primary focus in Make Up Shake Up and Raise This Roof which are both set during the first film.
In Epic Fails, Applejack recalls her most embarrassing moment when she and Pinkie Pie accidentally set off sprinklers at her house after burning pies in the oven. She has supporting or minor roles in A Photo Booth Story, Monday Blues, Pet Project, Get the Show on the Road, and Good Vibes. She also appears in photographs in Leaping Off the Page and Mad Twience and on Daring Do movie posters in The Canterlot Movie Club.
Applejack is a main focus in Queen of Clubs, Aww... Baby Turtles, Lost and Found, Blue Crushed, Turf War, and Five to Nine. She also makes supporting or minor appearances in A Fine Line, Overpowered, The Finals Countdown, My Little Shop of Horrors, Super Squad Goals, Road Trippin, The Last Day of School, Outtakes, and So Much More to Me.
In the second season, Applejack hosts a do-it-yourself video building Rarity's dressing room in DIY with Applejack. In Street Chic, Rarity redresses her in summer clothing, causing her to shiver in the cold fall air. In Best in Show: The Pre-Show and Best in Show: The Victory Lap, she and Fluttershy host and film the beginning and the end of the first annual Canterlot Pet Show. In Camping Must-Haves, she hosts an internet video with Rarity on essentials for camping for the Starswirled Music Festival and questions the practicality of Rarity's items. She also makes supporting or minor appearances in Schedule Swap, FOMO, and I'm on a Yacht; the music festival shorts Festival Filters, Festival Looks, and Five Lines You Need to Stand In; and Cheer You On and Do It For the Ponygram!.
Applejack is the main focus in Constructive Criticism, in which she is the set designer for the school play and recruits an assistant set designer to help her finish a set after sustaining a hand injury. She is a possible ending in Fluttershy's Butterflies, Driving Miss Shimmer, Rarity Investigates: The Case of the Bedazzled Boot, Opening Night, and Happily Ever After Party. She also appears in the Twilight Sparkle and Pinkie Pie endings of Best Trends Forever and makes a cameo appearance in Text Support. She is also mentioned in Stressed in Show.
In the second season, Applejack is a main focus in Accountibilibuddies, in which she injures her foot at the Starswirled Music Festival and her friends offer to come with her to see Dirk Thistleweed's performance. She is also a possible ending in the music festival shorts Wake-Up! and Inclement Leather as well as Tip Toppings and Costume Conundrum.
In Forgotten Friendship, Applejack and her friends are members of the Canterlot High Yearbook Club. Along with the rest of the school, her good memories of Sunset Shimmer are erased by Wallflower Blush using the memory stone, causing her to forget her friendship with Sunset and only remember her as a bully. The girls accordingly act with hostility towards Sunset for much of the special while enjoying their friendships with each other. After seeing Sunset jump in front of a blast from the memory stone intended to erase their memories of high school, the Equestria Girls use their geode powers to destroy the memory stone and restore the school's memories. They later win the "Best Friends" yearbook superlative.
In Rollercoaster of Friendship, Applejack and Rarity both apply for summer jobs as "Caramel Apple Girls" at the new Equestria Land theme park. Only Rarity is hired as a fashion designer for Vignette Valencia's light parade, for which the Rainbooms get booked as the centerpiece. For much of the special, Applejack gets jealous of Rarity's friendship with Vignette. When she later discovers Vignette using her magic phone to digitize her friends, Applejack tries to warn the rest of them about Vignette's true nature, but they dismiss her for being jealous, resulting in a falling-out with Rarity. After Rarity realizes Applejack was telling the truth, they make amends, rescue their friends, and stop Vignette's schemes, eventually taking their place as the parade's headlining act.
In Spring Breakdown, Applejack goes on a sea cruise with her friends for spring break, but she spends most of it seasick, exacerbated by Rainbow Dash's eagerness to fight Equestrian magic. When the ship's power fails, Applejack helps the crew fix the engines, inadvertently causing it to speed out of control and crash into rocks. After Twilight, Sunset, and Rainbow quell a magic thunderstorm born from the Storm King's magic, Applejack and her friends use their powers to evacuate the other passengers from the sinking ship. When they end up stranded on a nearby island, Sunset leads them an alternate way home through a portal to Equestria, where Applejack briefly turns into an Earth pony.
In Sunset's Backstage Pass, Applejack attends the Starswirled Music Festival with her friends. On the first day, Fluttershy accidentally spits juice on her as she tells a funny story at breakfast, and she roasts marshmallows with her friends in the evening. She is unknowingly caught in a time loop and repeats that day for the next three weeks. In one repeat, when Sunset tries to break the time loop by leaving the festival grounds and damages the RV, Applejack tells her of the damage and the costs to tow it. In another, she and her friends are kicked out of the festival when Pinkie provokes a security guard. In the final repeat, she and her friends spectate PostCrush's concert as they perform with Sunset and Pinkie.
In "Blizzard or Bust", Applejack and her friends attempt to fool Principal Celestia into declaring a snow day. In "Saving Pinkie's Pie", she takes part in a snowball fight. In "The Cider Louse Rules", she and Twilight team up to foil the Flim Flam brothers. In "Winter Break-In", after Sunset leaves her storage space key in her locker at school, Applejack suggests using their powers to retrieve it. In "Dashing Through the Mall", Applejack gives receives a gold hammer from Fluttershy as part of a Secret Santa-like gift exchange. In "O Come, All Ye Squashful", she accidentally ropes her friends into taking an embarrassing holiday photo with food costumes, and she ends up having to wear a costume of her own.