Are there Pony Vocaloids?

[EmeraldStar04 250]

I want a Twilight Sparkle vocaloid.

[Gamer_KM 90]

Vocaloid, no, mostly because Yamaha & hasbro are a pain with trademarks, however, Utau is a freeware version and you could easly pull samples from the show and make them a voice bank to use. Google around, see what comes up.