During my rewatch, I actually kept a Spike Owned counter to see how much unnecessary abuse he was given (if he agreed to the abuse beforehand, the counter doesn't go up, like helping Applejack in Fall Weather Friends, but when Rainbow hits him a few seconds later with no warning, the counter dings)
By the end of season three, the counter was at about 50, many of them from Twilight, whether it's slamming him with a door, pulling him around with magic, or throwing him/things at him when she gets excited - most common example is trampled, or crushed by various characters/things
Thankfully from S4 on, it dropped pretty sharply, and the final count was 75, with Applejack clobbering him with her luggage at the start of Rainbow Roadtrip
Poor guy deserved better, but that's often the fate of being a boy in a girl's cartoon